Amplification of unknown RNAs and RNA mixtures based on unique restriction enzyme cleavage in vitro
نویسندگان
چکیده
منابع مشابه
Cleavage-based signal amplification of RNA.
RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detec...
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the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
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Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events. Restriction enzyme cleavage and subsequent heat denaturation of a DNA sample generates two single-stranded target DNA fragments (T1 and T2). Present in excess are two DNA amplification primers (P1 and P2). The 3' end of P1 binds to the 3' end of T1, forming a duplex with 5' overha...
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ژورنال
عنوان ژورنال: Acta Biochimica et Biophysica Sinica
سال: 2010
ISSN: 1745-7270,1672-9145
DOI: 10.1093/abbs/gmq098